TABLE 1.
Morbidity of mice infected with wild-type and ΔHVR2 virusa
| Virus | Dose (PFU) | No. of mice:
|
Overall % morbidity | ||
|---|---|---|---|---|---|
| In group | With wt lossb | With clinical signsc | |||
| Mock | 4 | 0 | 0 | 0 | |
| Wild type (Alb240) | 5 × 103 | 8 | 7 | 8 | 100 |
| 5 × 104 | 8 | 8 | 8 | 100 | |
| 5 × 105 | 8 | 6 | 8 | 100 | |
| ΔHVR2 (Alb519) | 5 × 103 | 8 | 3 | 0 | 38 |
| 5 × 104 | 8 | 2 | 0 | 25 | |
| 5 × 105 | 8 | 3 | 0 | 38 | |
a
Six-week-old, female BALB/c mice were inoculated with virus or diluent. Mice were weighed and observed for clinical signs daily for 29 days. All mice survived. All mice inoculated with virus seroconverted; mock-inoculated mice remained seronegative.
b
Number of mice with ≥5% weight loss.
c
Number of mice with clinical signs, which included ruffled fur, hunching, aggression, “oily” fur, and circling (one mouse). Two mice in wild-type groups still had “oily” fur on day 29.