FIG. 5.

Time course analysis of viral RNA accumulations. HeLa-MHVR cells were infected with the indicated recombinant viruses at a multiplicity of infection of 0.01 PFU/cell, and total cellular RNAs were harvested from individual cultures at 4, 6 and 8 h postinfection. MHV N gene-specific RNAs were then quantified by RT-qPCR, normalizing the level of N gene amplicons to that of HeLa cell HPRT amplicons, as described in Materials and Methods.