TABLE 1.

Relative specific infectivities of rJ.6 and rJ.6-KO preparations

Expt	Virusa	Infectivity (PFU/ml)b	CT MHVc	CT VSVd	Relative MHV RNA contente	Relative specific infectivityf
1	rJ.6	1.1 × 106	24 (1.62-fold)	16.1 (0.87-fold)	1.86	1.18
	rJ.6-KO	5 × 105	24.7	15.9	1	1
2	rJ.6	2 × 105	24 (7.46-fold)	18.1 (1.62-fold)	4.6	0.87
	rJ.6-KO	5 × 104	26.9	18.8	1	1

^aIn two independent experiments, rJ.6 and rJ.6-KO were grown in parallel and collected from infected 17cl1 cell supernatants at 18 h postinfection.

^bInfectivities were determined by plaque assays on 17cl1 indicator cells.

^cC_T values for each virus pair were determined by RT-qPCR, and the differences in the C_T values were converted to the changes in the differences (n-fold), using 2^ΔCT = change in difference (n-fold).

^dC_T values for the VSV RNA extracted from the VSV-spiked samples were determined by parallel RT-qPCR, and the C_T differences were converted to the changes in the differences (n-fold).

^eRelative MHV RNA contents were determined by dividing the change in MHV RNA levels by the change in control VSV RNA levels. MHV RNA contents were normalized relative to rJ.6-KO.

^fRelative specific infectivity values (PFU/unit of genomic RNA) were determined by dividing the PFU ratios by the RNA ratios for each virus pair.