FIG. 1.

Rapamycin treatment rescues myxoma virus replication and spread in type II (restrictive) tumor cell lines. The ability of myxoma virus to replicate and spread following inoculation at a low MOI was determined by multistep growth curve using restrictive cancer cell lines 786-0 (renal cancer) (A and B), HCT116 (colon cancer) (C and D) and ACHN (renal cancer) (E and F). Wild-type virus vMyxlac (A, C, and E) and the M-T5 knockout virus vMyxT5KO (B, D, and F) were used to investigate the abilities of both viruses to infect and spread throughout the monolayer, and virus titer was assessed by focus formation. Cells were pretreated for 6 h before infection with 20 nM rapamycin (dashed line) or an appropriate vehicle control (1:5,000 dilution of DMSO, solid line). *, P, <0.005 by two-tailed t test.