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Rapamycin treatment increases focus size and number in type II cells. BGMK (A) or 786-0 (B) cells were infected with vMyxlac (top panels) or vMyxT5KO (bottom panels) at low MOIs. Cells were pretreated for 6 h before infection with 20 nM rapamycin or an appropriate vehicle control (1:5,000 dilution of DMSO). At 24 h postinfection, cellular lysates were collected, and released virus was used to infect fresh BGMK cells (shown). Foci are visualized by staining with X-Gal reagent.
