FIG. 5.

Rapamycin treatment did not alter myxoma virus replication in rabbit fibroblasts and lymphocytes. The ability of myxoma virus to replicate and spread following inoculation at a low MOI was determined by growth curves, using RK-13 (rabbit fibroblast cell line) (A and B) and RL-5 (rabbit lymphocyte cell line) (C and D). vMyxlac (A and C) and vMyxT5KO (B and D) were used to investigate the abilities of virus to infect and spread throughout the monolayer, and virus titer was assessed by focus formation. Cells were pretreated for 1 to 6 h before infection with 20 nM rapamycin (dashed line) or an appropriate vehicle control (1:5,000 dilution of DMSO, solid line). *, P, <0.005 as determined by two-tailed t test.