FIG. 8.

Effects of proteasome inhibitors on viral structural protein levels. Caco-2 cells infected with rotavirus strain RF (10 PFU/cell) for 12 h were subjected to proteasome inhibitor treatment with either ALLN (10 μM), MG132 (10 μM), or vehicle (lanes C) for the last 2 h of infection. Cells were harvested, and cell lysates were analyzed by immunoblotting using monoclonal anti-VP2, -VP6, and -VP4 as described in Materials and Methods. Note that treatment with proteasome inhibitors resulted in significant increases of viral structural protein levels, as follows: for VP2, 2.1-fold with ALLN and 2.8-fold with MG132; for VP6, 2.2-fold with ALLN and 2.4-fold with MG132; and for VP4, 2.3-fold with ALLN and 3-fold with MG132.