TABLE 2.
Blockade of FCoV and HCoV-229E infection with the anti-fAPN RG4 MAb in cells expressing fAPN or chimeric m/fAPN proteinsa
| APN protein | HCoV-229E infection
|
FCoV infection
|
||
|---|---|---|---|---|
| Control MAb | RG4 MAb | Control MAb | RG4 MAb | |
| fAPN | − | + | − | + |
| m/fAPN251-967 | − | + | − | + |
| m/fAPN251-582 | − | + | NA | NA |
| m/fAPN582-967 | NA | NA | − | −b |
| m/fAPN1-582 | − | + | NA | NA |
a
Infection with FCoV or HCoV-229E was detected by immunofluorescence with antiviral antibodies 16 to 18 h after inoculation. +, antibody blockade of virus infection (<5% of cells infected); −, no antibody blockade of virus infection (≥90% of cells infected). NA, not applicable; the m/fAPN chimeric protein has no receptor activity for the virus.
b
This antibody does not bind to this m/fAPN chimera.