TABLE 2.
Neutralization endpoints for 2909 against Envs with variant sequences in the V1/V2 domain
Clade B consensusb | V2 sequence at positiona | IC50 (μg/ml) | IC90 (μg/ml) |
---|---|---|---|
160167 | |||
GEIKNCSFNITTSIRDKVQKEYALFY | |||
SF162 | --------KV-----N-M-------- | 0.00019 | 1.1 |
SF-NI (K160N/V161I) | ---------------N-M-------- | >20 | >20 |
SF-GKV (N167G/M169V) | --------KV-----G---------- | 1.3 | >20 |
SF-NKV (M169V) | --------KV-----N---------- | 0.0088 | 5.5 |
SF-DKV (N167D/M169V) | --------KV---------------- | 0.000078 | 0.010 |
SF-DKM (N167D) | --------KV-------M-------- | 0.000080 | 0.0031 |
JR-FL | ----------------E--------- | >100 | >100 |
JR-FL (N160K) | --------K-------E--------- | 25 | >100 |
JR-FL (E168K) | -------------------------- | >20 | >20 |
JR-FL (N160S/E168K) | --------S----------------- | 0.70 | 6.3 |
JR-FL (N160K/E168K) | --------K----------------- | 0.00034 | 0.0093 |
SF162 (JR-FL V1V2 N160K/E168K) | --------K----------------- | 0.00012 | 0.0030 |
YU2 | -------------------------- | >20 | >20 |
YU2 (N160K) | --------K----------------- | 0.079 | 6.8 |
YU2 (T133A/N160K)c | --------K----------------- | 0.0053 | 2.9 |
V2 residues 152-177 (based on the HXB2 numbering system) are compared to the clade B consensus sequence. Dashes indicate residues identical to those of the consensus sequence. N-linked glycosylation signals [NX(S/T)] are underlined.
Env variants are described by listing in parentheses the original residue, the position, and the modified residue. For SF162 variants, a shorter name corresponding to that used in Fig. 1 is also provided in which the residues at key positions 160 to 161 or 167 to 169 are indicated. IC50 and IC90 values are averages of at least three independent assays.
See Table 3 for the YU2 V1 region sequence.