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IFN-α induction by CD46 Ads is pH dependent. PBMC were exposed to 104 viral particles/cell in the absence or presence of various concentrations of the endosomal pH inhibitors bafilomycin A1 (Baf), chloroquine, and ammonium chloride (NH4Cl) for 48 h. Culture supernatants were analyzed for IFN-α production by ELISA.
