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TRP-185 directly interacts with Rep68. rTRP-185 (1.2 pmol) was coupled to Ni-NTA beads and incubated with 3.75 pmol of rRep68 in the absence (−) or presence (+) of 2.5 pmol of mnAAVS1 wild-type (w) or mutant (mt) DNA. Eluted samples and input material were subjected to immunoblot analysis using anti-Flag and anti-His antibodies for rRep68 and rTRP-185, respectively.
