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Activation of damage proteins at late times p.i. requires active viral replication. (A) Top panels show localization of BrdU-labeled genomes inside the nucleus of T98G cells at early times p.i. Bottom panels show expression of viral antigen IE1 in approximately 30% of cells at late times p.i. (B) Immunoblot analyses of several damage response proteins in the semipermissive T98G cells. An actin loading control is included. For definitions of abbreviations, see the Fig. 1 legend.
