FIG. 8.
Rotavirus and cytokeratin 7 immunohistochemistry of the extrahepatic biliary ducts harvested from mice injected with the different strains of rotavirus. Dual-staining immunohistochemistry was performed on the extrahepatic biliary ducts harvested after inoculation with the five strains of rotavirus. The sections were harvested at day 5 following injection in all mice, except those injected with SA-11FM, which were harvested at day 2. The sections underwent indirect immunofluorescence for cytokeratin 7 and rotavirus as described in Materials and Methods. Cytokeratin 7 is found in the biliary epithelial cells. Column 1, FITC-cytokeratin 7 (green); column 2, Texas Red-rotavirus (red); column 3, merged image column 1 and 2. Colocalization of virus to biliary epithelium appears as orange staining. Magnification, ×20.