TABLE 1.
Rotavirus proteins in tissue extracts from mice inoculated with the five different strainsa
Organ | Day postinjection | Amtb of rotavirus protein from strain:
|
||||
---|---|---|---|---|---|---|
RRV | SA11-FM | SA11-SM | EDIM | Wa | ||
Liver | 2 | 1.48 ± 0.43 | 3.31 ± 0.14 | 0.86 ± 0.39 | 2.14 ± 0.64 | 6.17 ± 1.25 |
5 | 7.72 ± 2.04 | 2.16 ± 0.94 | — | 5.00 ± 1.32 | 0.45 ± 0.20 | |
7 | 4.47 ± 0.46 | 6.53 ± 0.93 | 5.84 ± 1.02 | 6.76 ± 1.86 | — | |
10 | 56.04 ± 16.55 | 4.31 ± 0.71 | 0.47 ± 0.20 | 0.82 ± 0.29 | 0.02 ± 0.02 | |
14 | 0.64 ± 0.11 | 1.55 ± 0.89 | 0.21 ± 0.13 | — | — | |
Intestine | 2 | 1.19 ± 0.08 | 1.66 ± 0.09 | 0.11 ± 0.11 | 1.10 ± 0.16 | 4.15 ± 0.78 |
5 | 1.00 ± 0.38 | 0.14 ± 0.06 | 1.35 ± 0.83 | 3.94 ± 0.68 | 0.22 ± 0.08 | |
7 | 1.09 ± 0.20 | 0.30 ± 0.11 | 0.04 ± 0.02 | 3.59 ± 1.13 | 0.08 ± 0.08 | |
10 | 0.16 ± 0.04 | 0.30 ± 0.20 | — | 10.59 ± 2.98 | — | |
14 | —c | 0.03 ± 0.03 | — | 0.52 ± 0.15 | — | |
Spleen | 2 | — | — | — | 13.39 ± 2.46 | 14.57 ± 6.72 |
5 | 1.88 ± 0.95 | 0.53 ± 0.33 | 0.36 ± 0.36 | 3.58 ± 0.56 | 0.81 ± 0.54 | |
7 | 1.93 ± 0.42 | 1.96 ± 0.92 | — | 9.18 ± 2.19 | — | |
10 | 3.93 ± 0.98 | — | — | — | — | |
14 | — | — | — | — | — | |
Kidney | 2 | — | 0.08 ± 0.12 | — | 11.22 ± 2.41 | 7.83 ± 2.59 |
5 | 0.24 ± 0.12 | 0.25 ± 0.25 | 0.34 ± 0.22 | 1.18 ± 0.18 | 0.50 ± 0.31 | |
7 | 0.97 ± 0.20 | 0.20 ± 0.06 | — | 6.17 ± 2.52 | — | |
10 | 1.13 ± 0.27 | 0.08 ± 0.08 | — | 0.53 ± 0.26 | — | |
14 | — | — | — | — | — | |
Brain | 2 | — | — | — | 2.41 ± 0.46 | — |
5 | — | — | — | 0.01 ± 0.01 | — | |
7 | 0.02 ± 0.01 | 0.02 ± 0.01 | — | — | — | |
10 | 0.03 ± 0.03 | — | — | — | — | |
14 | — | — | — | — | — |
Groups of ≥10 pups per time point were injected with 1.5 × 106 FFU of the five strains of rotavirus. Organs were harvested on the day postinfection indicated and homogenized, and the presence of rotavirus protein was determined by ELISA.
Values are expressed as mean ng/mg of tissue weight ± standard errors.
—, value below the limit of detection.