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Residual antigen present in the DC-rich regions of lymph nodes drives proliferation of naïve cells. CFSE-labeled CD45.1 OT-I cells (1.25 × 106) were transferred i.v. into CD45.2 B6 mice 21 days after intranasal infection with VSV-Ova or VSV-Ind. Cervical LN were harvested from animals 4 days after transfer, reacted with MAb specific for CD11c (red) and CD45.1 (blue), and then analyzed by confocal microscopy using a 10× water objective. Cells with CFSE remaining appear green.
