TABLE 2.
Primers for real-time QPCR p53 target gene analysis
| Target | Sense primer (5′ to 3′) | Antisense primer (5′ to 3′) | Size (bp) |
|---|---|---|---|
| p53 | AGGCCTTGGAACTCAAGGAT | TGAGTCAGGCCCTTCTGTCT | 140 |
| p21 | GGAAGACCATGTGGACCTGT | GGCGTTTGGAGTGGTAGAAA | 146 |
| PUMA | GACGACCTCAACGCACAGTA | CTGGGTAAGGGCAGGAGTC | 113 |
| Bcl-2 | ATGTGTGTGGAGAGCGTCAA | ACAGTTCCACAAAGGCATCC | 136 |
| Bax | GGGGACGAACTGGACAGTAA | CAGTTGAAGTTGCCGTCAGA | 122 |
| Bad | CGGAGGATGAGTGACGAGT | CCACCAGGACTGGAAGACTC | 123 |
| APO-1 | ATAAGCCCTGTCCTCCAGGT | TGGAAGAAAAATGGGCTTTG | 120 |
| KILLER | GATGGTCAAGGTCGGTGATT | CCCCACTGTGCTTTGTACCT | 79 |
| cdc25c | TCCTTAAAGGCGGCTACAGA | GGGCAGTAGCTCTGTGGTTC | 70 |
| EZH2 | TTCATGCAACACCCAACACT | CTCCCTCCAAATGCTGGTAA | 96 |
| p53R2 | GCCAGGACTCACTTTTTCCA | TCAGGCAAGCAAAGTCACAG | 71 |
| PIG3 | ACGCTGAAATTCACCAAAGG | AACCCATCGACCATCAAGAG | 107 |
| Gadd45 | GGAGGAAGTGCTCAGCAAAG | ATCTCTGTCGTCGTCCTCGT | 135 |
| 14-3-3α | ACCCAATTCGTCTTGGTCTG | GCTTCATCAAATGCCGTTTT | 105 |
| Glyceraldehyde-3-phosphate dehydrogenase | CCAGGTGGTCTCCTCTGACTTCTC | ATACCAGGAAATGAGCTTGACA | 107 |