FIG. 3.

VP4 and caveolin expression in DRM from MA 104 cells. MA 104 cells were infected and labeled with [³H]cholesterol as described in the legend to Fig. 2. Cells were then lysed in TNE buffer containing 1% of one of the following detergents: TX-100, Lubrol-WX (Lubrol), Nonidet P-40 (NP-40), or Brij 58. After flotation on a sucrose gradient, 1-ml fractions were collected from the top. (A) Fractions 4 and 5, corresponding to floating material, were analyzed by SDS-PAGE and Western blotting for the virus protein VP4, flotillin (Flot), and caveolin-1 (Cav-1). (B) Radioactivities in fractions were counted, and cholesterol content is expressed as disintegrations per minute (dpm) for individual fractions.