FIG. 5.

DRM in the two cell lines essentially differ by the total amount of lipids. DRM were collected from MA 104 cells and from Cav-1-transfected and wild-type Caco-2 cells and were analyzed for cholesterol (Chol.), sphingomyelin (SM), and glycerophospholipid (GPL) contents as described in Materials and Methods. Data are expressed in nmol of lipids recovered in DRM per mg of cell protein initially layered on the gradient and are means (n = 6 for Caco-2 cells, n = 3 for MA 104 cells, and n = 2 for Cav-1-expressing Caco-2 cells).