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MKlp2 BPV E2-binding region mapping. Extracts of C33a cells cotransfected with BPV E2 and a series of Flag-tagged MKlp2 fragment expression constructs were immunoblotted and probed by anti-Flag M2. Immunoprecipitations were performed by using B201 or anti-EE (α-EE) monoclonal antibody as a control. HC, heavy chain; LC, light chain.
