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. 2006 Nov 29;81(4):1736–1745. doi: 10.1128/JVI.01638-06

FIG. 4.

FIG. 4.

Characterization of anti-MKlp2 antibody. (A) Fifty micrograms of HeLa cell extracts synchronized using a double-thymidine block were blotted and probed by affinity-purified anti-MKlp2. The same cell extracts were probed for cyclin B1 (Sc-245; Santa Cruz) on the bottom panel. S, S phase; M, mitotic cell lysate. (B) Extracts of C33A cells cotransfected with Flag-tagged full-length MKlp2 plus Flag-MKlp2N (aa 1 to 499) or MKlp2C (aa 496 to 886) followed by immunoprecipitation with anti-MKlp2 antibody were blotted and probed with anti-Flag M2.