FIG. 5.
HPV E2 protein complex with MKlp2. (A) CV-1 cells with inducible Flag-HPV-11 E2 were transfected with the Flag-MKlp2 expression construct. Four hours before harvesting, expression of HPV-11 E2 was induced by 2 μM CdSO4 in complete medium. Anti-MKlp2 (αMKlp2) and antigen-preabsorbed antisera (Ctrl Ab) were used for immunoprecipitations. Immunoprecipitates were blotted and probed with anti-Flag M2 (α Flag). (B) U2OS cells with or without stably expressed HPV-16 E2 were synchronized using a double-thymidine block. Extracts were blotted and probed with TVG261 (top panel) to demonstrate E2 expression. Anti-MKlp2 and antigen-preabsorbed antisera (Ctrl Ab) were used for immunoprecipitation. Precipitates and cell extracts as input were blotted and probed with anti-MKlp2 and TVG261, respectively. S, S phase; M, mitotic cell lysate.