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Curing the 154-kb plasmid from Pph race 7 strain 1449B. (A) Plasmid profiles from races of Pph; M gives profile from E. coli 39R861 that contains plasmids of known size (44). (B) The 154-kb plasmid pAV511 was cured from 1449B by using the rep gene cloned in pPPY51. Both RW50 and RW60 have been cured of pAV511, but RW50 retains pPPY51. The smaller plasmid in 1449B, RW50, and RW60 is designated pAV512. Chr, chromosomal DNA.
