Reaction phenotypes in bean pods and soybean leaves inoculated with Pph. (A) Pods of bean cultivars (from left to right): A43, Canadian Wonder, Red Mexican, and Tendergreen 3 days after stab-inoculation with (top to bottom site): Pph race 7 strain 1449B, cured strain RW60, complemented strains RW60 (pAV521) and RW60 (pAV518), and RW60 (pLAFR3) vector control. Note that pAV521 carries avrPphF, the avr gene matching resistance gene R1 in Red Mexican. (B) Time course of HR development in pods of cv. Red Mexican photographed 1, 2, 3, and 5 days after inoculation with 1449B (Upper) and RW60 (Lower); note the more orange lesion caused by the plasmid-cured strain. (C) Leaves of soybean cv. Osumi 10 days after inoculation with (left to right) 1449B, RW60, and RW60 (pAV518).