Figure 5. HPC offers in vivo protection to mice by decreasing inflammation in lungs.

(A) Whole left lungs from mice were harvested following protocols of normoxia; severe hypoxia; HPC followed by severe hypoxia. Wild-type lungs display a macroscopic increase in tissue volume only in hypoxic conditions. Scale bar: 1 cm. Histology of the lungs shows noticeable hemorrhage in the hypoxic condition, while the lungs of preconditioned mice display histology similar to that of control, normoxic lungs. Scale bar: 50 μm. (B–D) Low-power magnification. (E–J) Higher-power magnification shows normoxic histology (B and E) and normal artery size (arrow) and periarterial space. Hypoxic lung displaying perivascular hemorrhage (C and F) and dilated artery and periarterial cuffing (I). HPC followed by hypoxia (D, G, and J) showing histology similar to that in normoxia.