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. 2007 Feb 16;3(2):e20. doi: 10.1371/journal.ppat.0030020

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© 2007 Stiasny et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Coflotation of virus with liposomes at alkaline pH. Virus was incubated for 10 min with liposomes at 37 °C at pH 10.0 (boxes) and as controls at pH 5.4 (diamonds) or pH 8.0 (circles), back-neutralized, and then subjected to centrifugation in sucrose step gradients. The gradients were fractionated, and the amount of E protein in each fraction was determined by a quantitative four-layer ELISA after denaturation of the samples with 0.4% SDS. The top fractions containing virus bound to liposomes are indicated by a bracket.

(B) Percentage of E protein bound to liposomes at different pHs compared with the control at pH 5.4.

(C) Percentage of E protein bound to liposomes at pH 10.0 after preincubation with different monoclonal antibodies compared with the control without monoclonal antibodies.