FIG. 3.
Effects of simultaneous treatment or posttreatment with GFLX on IL-8 secretion from PC-3 cells. PC-3 cells were plated at 2 × 105 cells in a 24-well plate. After 24 h of incubation, GFLX (16 μg/ml) was added simultaneously (GFLX at 0 min) with TNF-α stimulation (0, 5, 10, and 25 ng/ml) or 30 min or 60 min after TNF-α stimulation (GFLX at 30 min and GFLX at 60 min, respectively). The cells were incubated with TNF-α for 120 min. The concentrations of IL-8 secreted into the medium were determined by an enzyme-linked immunosorbent assay, as described in Materials and Methods. The data shown are the means ± SDs for three experiments.