FIG. 4.
GFLX decreases IL-8 mRNA expression. (A) PC-3 cells were plated at 4 × 105 cells in a 35-mm dish. After 24 h of incubation, the cells were treated with 16 μg/ml GFLX for 48 h. TNF-α at 10 ng/ml was then added, and the cells were incubated for an additional 4 h. Total RNA was isolated, and RT-PCR was performed as described in Materials and Methods. After electrophoresis, the PCR products were visualized by ethidium bromide staining. (B) Densitometric analysis of the bands of PCR products shown as the ratio of IL-8/β-actin. P was <0.001 for the results for cells incubated with GFLX compared with the results for cells incubated without GFLX.