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ATP-mediated excision of AZT-MP and rescue of DNA synthesis carried out by WT, Aztr (M41L/L210W/T215Y), and Aztr K70E RT. (A) ATP-mediated unblocking reaction analyzed by denaturing gel electrophoresis. Excision products were analyzed at 4, 8, 12, 16, 20, 40, 60, and 90 min. (B) Isotherms for ATP-mediated phosphorolytic excision of AZT-MP by WT (ο), Aztr (▿), and Aztr K70E (□) HIV-1 RT. Data are the means of three independent experiments.
