FIG. 2.
AmPrβCD inhibits internalization rather than binding of LFn-Al to PA/ANTXR complex. Cells were plated on glass coverslips at 105 cells/coverslip and 20 h later were shifted to fresh culture medium (no PA), medium supplemented with 8 nM PA alone (PA), or PA in combination with 100 μM AmPrβCD (PA + Drug) and preincubated for 15 min at 37°C. LFn-Al was added to a final concentration of 2 nM, and cells were incubated for 1 h at 37°C (A) or 4°C (B). Blue, DAPI nuclear counterstaining; red, AlexaFluor-594. Scale bars, 20 μm.