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Ability of albicidin (Alb) to stabilize the gyrase-DNA cleavage complex in the presence or the absence of ATP or ADPNP and with relaxed or supercoiled DNA substrate. O, open circle DNA; L, linear DNA; C, closed circle DNA. The formation of linear DNA indicates stabilization of the cleavage complex. The DNA gyrase concentration was 34 nM. The reaction mixtures for the control panel did not include either ATP or ADPNP.
