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Screening of the PKS gene-disrupted mutants by PCR assays. Genomic DNA was prepared from the gentamicin-resistant colonies and used as the template for PCR with primers P4 and T7 (Fig. 2). The expected size of the PCR product from homologous recombinants is 522 bp. Lane 1, control reaction without template; lane 2, wild type; middle unnumbered lanes, gentamicin (Gm)-resistant colonies; lane 4, 100-bp DNA ladder.
