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. 2006 Oct 30;51(1):64–72. doi: 10.1128/AAC.00931-06

FIG. 5.

FIG. 5.

Partial map of the 13.5-kb region of L. enzymogenes strain C3 hosting the HSAF biosynthetic genes. The predicted functions of the open reading frames are indicated. Restriction enzyme sites: A, ApaI; B, BamHI; E, EcoRI; K, KpnI; S, SacI. Domains of the hybrid PKS-NRPS: KS, ketosynthase; AT, acyltransferase; DH, dehydratase; KR, ketoreductase; ACP, acyl carrier protein; C, condensation; A, adenylation; PCP, peptidyl carrier protein; TE, thioesterase. The highly conserved signature motifs within the domains are shown, with the active-site residues highlighted in boldface letters. MxaD, PKS from Stigmatella aurantiaca (GenBank accession number AAK57188); MxaE, PKS from S. aurantiaca (GenBank accession number AAK57189); HSAF, the PKS-NRPS from L. enzymogenes C3; GenBank accession number YP_529192, PKS-NRPS sequence from Saccharophagus degradans 2-40 (GenBank accession number YP_529192).