FIG. 2.

Histopathological analysis of antifungal prophylaxis for systemic zygomycoses. The numbers of infection foci (clusters of hyphae) in mice infected with R. oryzae 4570 (A) and A. corymbifera 4535 (B) and the sizes of the infection foci in mice infected with R. oryzae 4570 (C) and A. corymbifera 4535 (D) are shown. Brains and kidneys were retrieved 3 days postinfection from control mice (n = 5; black histograms), posaconazole-treated mice (n = 5; white histograms), and amphotericin B-treated mice (n = 5; gray histograms). Tissue sections were prepared and stained with Grocott-Gomori's methenamine-silver nitrate. At least 20 microscopic fields were examined for each mouse, and the number of infection foci and their sizes were determined. Data are reported as the means ± standard errors of the means. Analysis of variance with Bonferroni's correction was used for statistical analysis. Asterisks indicate a significant difference between the treated and the untreated groups (P < 0.05).