TABLE 1.
MICs of various aminoglycosides against recombinant E. coli DH5α producing or not producing S. maltophilia APH(3′)-IIc and against S. maltophilia K279a and an aphFS derivative
| Strain or recombinant and condition | MIC (mg/liter) ofa:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| AMI | BUT | GENT | KAN | LIV | NEO | PARO | SIS | TOB | |
| DH5α::pBAD | |||||||||
| No arabinose | 0.5 | 0.125 | 1 | 0.25 | 4 | 0.5 | 0.25 | 0.125 | 0.5 |
| With arabinose | 0.5 | 0.125 | 1 | 0.25 | 4 | 0.5 | 0.25 | 0.125 | 0.5 |
| DH5α::pBAD[aph(3′)-II] | |||||||||
| No arabinose | 0.5 | 0.125 | 1 | 0.25 | 4 | 0.5 | 0.25 | 0.125 | 0.5 |
| With arabinose | 4 | 4 | 1 | >256 | 8 | 64 | 128 | 0.125 | 8 |
| K279a | 64 | 8 | 16 | 256 | 128 | 512 | 256 | 8 | 32 |
| K279a (aphFS) | 64 | 1 | 16 | 8 | 128 | 64 | 64 | 8 | 32 |
a
MICs were determined using agar dilution on Muller-Hinton agar. Abbreviations: AMI, amikacin; BUT, butirosin; GENT, gentamicin; KAN, kanamycin; LIV, lividomycin; NEO, neomycin; PARO, paromomycin; SIS, sisomicin; TOB, tobramycin.