FIG. 3.

hBD-2 killing of C. albicans is ion selective. C. albicans (10⁶ cells/ml) was treated with 11.6 μM hBD-2 (A) or 1.0 μM hBD-3 (B) in the presence of 10 mM sodium phosphate buffer (Na₂HPO₄-NaH₂PO₄, pH 7.4) supplemented with CaCl₂ (clear bars), MgCl₂ (gray bars), NaCl (black bars), or KCl (hatched bars) for 1 h at 30°C. Loss of cell viability is expressed as [1 − (colonies after peptide treatment/colonies after incubation with buffer only)] × 100. Each data point represents the mean ± the standard deviation of at least three independent experiments.