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. 2006 Oct 30;51(1):154–161. doi: 10.1128/AAC.00478-06

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Copyright © 2007, American Society for Microbiology

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FIG. 5. — Both C. glabrata and C. albicans have Ssa proteins in their cell wall and cytoplasmic fractions. Cell wall proteins were extracted from C. glabrata 931010 (lane 1) and C. albicans CAF4-2 (lane 2) by β-ME digestion. Cytosolic fractions of C. glabrata (lane 3) and C. albicans (lane 4) were obtained by glass bead disruption of β-ME-digested cells, and soluble proteins were recovered. All cellular fractions were normalized by protein content, and Western blots were probed with an anti-HSP70 monoclonal antibody.