TABLE 1.
Viable counts of bifidobacteria before and after acid, oxgall, or hydrogen peroxide challenge in MRS broth
| Organism | Viable count of bifidobacteria (log10 CFU/ml on MRS agar) upon challenge with:
|
|||||
|---|---|---|---|---|---|---|
| Acida
|
Oxgallb
|
H2O2c
|
||||
| 0 | 60 min | 0 | 90 min | 0 | 90 min | |
| B. animalis ATCC 27536 | 8.73 ± 0.07 | 7.37 ± 0.18 | 8.16 ± 0.11 | 7.94 ± 0.06 | 8.59 ± 0.05 | 8.21 ± 0.24 |
| B. bifidum R 0071 | 8.54 ± 0.06 | 3.70 ± 0.02 | 8.17 ± 0.08 | 8.14 ± 0.05 | 8.15 ± 0.15 | 7.17 ± 0.18 |
| B. bifidum ATCC 15696 | 7.25 ± 0.14 | <2.00 | 6.95 ± 0.02 | 6.84 ± 0.09 | 7.45 ± 0.26 | <2.00 |
| B. bifidum BB12 | 8.25 ± 0.19 | 6.07 ± 0.07 | 8.39 ± 0.08 | 8.13 ± 0.04 | 8.17 ± 0.07 | 8.19 ± 0.06 |
| B. breve ATCC 15700 | 8.25 ± 0.13 | 3.79 ± 0.10 | 8.79 ± 0.13 | 8.52 ± 0.15 | 8.62 ± 0.12 | 8.63 ± 0.04 |
| B. breve R0070 | 8.10 ± 0.17 | 3.69 ± 0.08 | 8.61 ± 0.15 | 8.36 ± 0.14 | 8.16 ± 0.11 | 7.63 ± 0.19 |
| B. infantis ATCC 15697 | 8.48 ± 0.28 | 3.99 ± 0.01 | 8.33 ± 0.35 | 7.46 ± 0.15 | 8.37 ± 0.18 | <2.00 |
| B. longum ATCC 15707 | 8.65 ± 0.24 | 3.40 ± 0.01 | 8.47 ± 0.07 | 8.11 ± 0.10 | 8.19 ± 0.08 | 8.00 ± 0.06 |
| B. longum ATCC 15708 | 8.09 ± 0.35 | <2.00 | 8.32 ± 0.20 | 8.19 ± 0.19 | 8.64 ± 0.07 | 8.01 ± 0.07 |
| B. longum R0175 | 8.39 ± 0.05 | 3.49 ± 0.04 | 8.92 ± 0.08 | 8.89 ± 0.10 | 8.70 ± 0.12 | 8.53 ± 0.23 |
| B. longum P/N 601377 | 8.05 ± 0.10 | 3.69 ± 0.04 | 8.33 ± 0.35 | 8.15 ± 0.18 | 8.30 ± 0.30 | 8.15 ± 0.03 |
| B. pseudolongum ATCC 25562 | 8.46 ± 0.15 | 7.78 ± 0.11 | 7.88 ± 0.08 | 7.46 ± 0.15 | 8.60 ± 0.11 | <2.00 |
| B. thermophilum ATCC 25866 | 8.37 ± 0.18 | 3.69 ± 0.08 | 8.09 ± 0.06 | 7.83 ± 0.07 | 8.27 ± 0.11 | 8.15 ± 0.06 |
a
MRS broth at pH 2.0, incubated anaerobically.
b
MRS broth plus 0.3% (wt/vol) oxgall, at pH 6.5, incubated anaerobically.
c
MRS broth plus H2O2 at the MIC, at pH 6.5, incubated anaerobically.