TABLE 2.
Antiviral susceptibilities of AD169-derived mutants assessed by PRAa
| Virus | DNA pol genotype | GCV
|
FOS
|
CDV
|
|||
|---|---|---|---|---|---|---|---|
| IC50 (μM) | Fold increaseb | IC50 (μM) | Fold increaseb | IC50 (μM) | Fold increaseb | ||
| AD169 | Wild type | 1.9 ± 0.5 | 78 ± 13 | 0.9 ± 0.3 | |||
| T2293 | N408K | 10 ± 3.0 | 5.3 | 122 ± 48 | 1.6 | 4.9 ± 1.3 | 5.4 |
| T2291 | A834P | 4.1 ± 2.0 | 2.2 | 431 ± 184 | 5.5 | 3.3 ± 1.3 | 3.7 |
| T2311 | N408K + A834P | 12.4 ± 1.8 | 6.5 | 732 ± 418 | 9.4 | 8.9 ± 4.4 | 9.9 |
a
Mean values and standard deviations were calculated from three replicate experiments.
b
Fold increase in IC50 compared to the average IC50 for wild-type sensitive strains.