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Characterization of the purified rNP5. Lanes: 1, 1 μg of purified rNP5 in silver-staining-based sodium dodecyl sulfate-polyacrylamide gel electrophoresis; 2, Western blot analysis of purified rNP5. SARS-positive serum (1:1,000) from a convalescent SARS patient was used for detection. Molecular mass marker M is shown on the left in kilodaltons.
