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. 2006 Nov 1;14(1):74–80. doi: 10.1128/CVI.00250-06

FIG. 1.

FIG. 1.

Visualization of virus-specific CD8+ T cells by staining ex vivo with MHC-I tetramers and surface expression of memory/effector phenotypic markers. All cells shown are a result of compound gating; an initial gate was set on the CD8high subset on CD8 and SS-log, followed by a lymph-gate on FS-area and SS-log. (A) Representative figure showing surface expression of CD27, CD28, CD57, and CD62L on EBV-, CMV-, and HIV-1-specific CD8+ T cells. Numbers in the quadrants indicate percentages of cells. (B) Comparison of percentages of CD27, CD28, CD57, and CD62L expressing EBV-, CMV-, and HIV-1-specific CD8+ T cells. Data are from the participants with EBV tetramer reactivity (n = 5), CMV tetramer reactivity (n = 9), and HIV-1 tetramer reactivity (n = 14).