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. 2006 Nov 1;14(1):74–80. doi: 10.1128/CVI.00250-06

FIG. 2.

FIG. 2.

HIV-1-specific CD8+ T cells do not show effector maturation-associated progression from the CD27high CD57− subset to the CD27− CD57high subset. (A) Proportions of four binary CD27 CD57 subsets of EBV-, CMV-, and HIV-1-specific CD8+ T cells are compared. The green line indicates the mean of each subset. Data are from the participants with EBV tetramer reactivity (n = 5), CMV tetramer reactivity (n = 9), and HIV-1 tetramer reactivity (n = 14). (B to E) Proportions of the CD27+ CD57+ (CD27high CD57low) subsets of HIV-1-specific and bulk CD8+ T cells positively correlated with HIV-1 plasma viral load (B and D) but not with absolute CD4 counts (C and E). HIV-specific CD8+ T cells from HIV-infected individuals (n = 7) are plotted against HIV-1 plasma viral load (log10VL). Mean values of corresponding percent CD27+ CD57+ of HIV-specific and bulk CD8+ T cells are used for those with multiple HIV-1-specific tetramer reactivity. (F) Representative three-dimensional topographical plots of CD27 and CD57 expression on EBV (panel i)-, CMV (panel ii)-, and HIV-1 (panels iii and iv)-specific CD8+ T cells are shown. Color variation indicates a number of cells from low (purple) to high (red) on a z axis. The white arrow indicates the CD27− CD57high subset. Percent CD27− CD57+ of representative results (colored circles) are shown in Fig. 2A. Panels i and ii represent EBV- and CMV-specific CD8+ T cells from HIV-1 uninfected (red circles, participants 9 and 8, respectively), while panels iii (green circle, B8nef from participant 1), and iv (blue circle, A3pol from participant 7) represent HIV-1-infected participants.