TABLE 1.
Summary of the characteristics of monoclonal antibodies
| MAb | Immunized antigena | Antigen reacted (kDa)b | Reaction against species (no. of strains tested)c
|
Agglutination activityd | Ig isotypee | ||
|---|---|---|---|---|---|---|---|
| F. tularensis (26) | F. novicida (1) | F. philomiragia (3) | |||||
| M11D3 | GIEM Miura | 40 | + | + | + | − | M |
| M11H7 | GIEM Miura | >15g | + | − | − | − | G3 |
| M13B10 | GIEM Miura | 17 | + | + | + | − | G1 |
| M14B11 | GIEM Miura | >15g | + | − | − | + | G2a |
| M15C6 | GIEM Miura | >15g | + | − | − | + | M |
| S11E7 | Schu | 10 | + | + | + | − | G1 |
| U22F2 | U112 | 41, 43 | + | + | − | − | G1 |
| FB11f | 15 | >15g | + | − | − | − | G2a |
a
Francisella strains used for immunization of mice.
b
Molecular mass of F. tularensis antigen appeared in Western blotting following SDS-PAGE.
c
Determined by indirect fluorescence assay: +, positive; −, negative.
d
Determined by microagglutination test: +, positive; −, negative.
e
Determined with a mouse monoclonal antibody isotyping test kit (Serotec, Oxford, United Kingdom).
f
Reference MAb purchased commercially.
g
Ladder-like bands of molecular mass greater than 15 kDa.