FIG. 1.

(a) Overview of affinity column purification scheme. Mouse anti-NPrGBMP-rPorB antisera were pooled and run over affinity matrices as shown. Bound antibodies were eluted from each resin with 3.0 M KSCN. (b) ELISA of affinity column fractions on a microtiter plate coated with native GBMP-HSA. (c) ELISA of the same affinity column fractions on a microtiter plate coated with modified NPrGBMP-HSA. Symbols: •, GCMP column; ▾, GBMP column; ▪, NPrGBMP column. OD 450 nm, optical density at 450 nm.