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Structures of vectors used in the Trofile assay. (A) Patient eETVs were constructed by cloning the amplified env genes from patient plasma samples into pCXAS-PXMX (see the text for details). The amplified fragment comprised the entire open reading frame of HIV-1 gp160. (B) A replication-defective genomic vector, RTV1.F-lucP.CNDOΔU3, was constructed with a luciferase cassette inserted into a deleted region of the env gene of the NL4-3 strain of HIV-1.
