FIG. 5.

Sensitivity to detect minor variants within mixed populations of clones derived from four patients (244, 417, 1354, and 2188). The results are displayed as median RLU from replicates (see the text for details); the error bars are 1 standard deviation from the median (see the text for details). (A) Mixture 244. Minority species of X4- and R5-tropic viruses were both detected in mixed viral stocks at the lowest frequency tested (5% of the population). (B) Mixture 417. Minority species of R5-tropic viruses were detected in mixed viral stocks at the lowest frequencies tested (5% of the population). The minority X4-tropic variant was detectable only when its frequency was greater than 10% of the total population. (C) Mixture 1354. The minority species of X4- and R5-tropic viruses were both detected in mixed viral stocks at the lowest frequency tested (5% of the population). (D) Mixture 2188. The minority species of X4- and R5-tropic viruses were both detected in mixed viral stocks at the lowest frequency tested (5% of the population).