FIG. 2.

Effect of VRT-125853 on DNA, RNA, and protein synthesis in S. aureus. Metabolite incorporation assays were performed as described in Materials and Methods. The synthesis of DNA (A), RNA (B), and protein (C) were measured in S. aureus cells exposed to five times the MIC of VRT-125853 or control compounds; and the levels of incorporation of [³H]thymidine (DNA synthesis), [³H]uridine (RNA synthesis) or ³H-amino acid mixture (protein synthesis) during the course of the experiment were measured and are expressed as a percentage of that for the untreated (no drug) control. Ciprofloxacin (CIP), novobiocin (NOV), erythromycin (ERY), rifampin (RIF), and gramicidin D (GRA-D) were the control compounds used for comparison. The results for VRT-125853 are shown as shaded bars.