TABLE 1.

Sequences of primers used for PCR amplification and DNA sequencing of gyrB and parE DNA encoding the ATP binding region of mutants

Primer name	DNA sequence
S. aureus gyrB forward	CGATTCAGCATAAAGTACAAACATTTGTCACTACGACATCTG
S. aureus gyrB reverse	CCGCCCTCATAGTGATAGGAGTCTTCTCTAACG
S. aureus grlB forward	GCATTTTACGCTGATTTATATAAGAATAACTATTGTATAGTTTTAAAAACGAAC
S. aureus grlB reverse	GCTCTTGACGCTCTTTACCACTGCGTAAATCATTAAGCG
E. faecalis gyrB forward	CGTCATTGTATTGATAAGCGACCTCTACTTCG
E. faecalis gyrB reverse	CGGGGATCCCTTCAATTGAAATGTGTAAGCCTCG
E. faecalis parE forward	CGCGGATCCGGCTCACTCTTCTTCTAATTC
E. faecalis parE reverse	CGGGGATCCGGGTTCTTCCCCACGTTCATC
S. pneumoniae gyrB forward	CGCTCTAGAGCTGCAGGACTTGATACCCACTTG
S. pneumoniae gyrB reverse	GCCTCTAGACAGTGATAGAAATTTGAAGACCGCG
S. pneumoniae parE forward	CGGTCTAGACTCTGCTGAAATTGTCACATCGGAG
S. pneumoniae parE reverse	CGGTCTAGACGCTTGTCCGTTAAAGACAAGGTCAC
H. influenzae gyrB forward	CGTTTTCCTTTCCCGAAAAGCATCGCCAAAACGGCG
H. influenzae gyrB reverse	CTACGAATGCTTGAATACCGCCTTCATAATGGAAATGATC
H. influenzae parE forward	GTGCGGTCGAATATCACCGCACTTTTTGATAG
H. influenzae parE reverse	CGGAAGGGTTTCAAAGCCATTTACTGCTTCG