TABLE 3.

Genetic proof that mutations in GyrB/ParE confer resistance to VRT-125853 and VRT-752586 in S. aureus and H. influenzae^a

Strain	Chromosome	Plasmid	MIC (μg/ml)				No. of transformants/ selection plate
			VRT-125853	VRT-752586	Novo	Cipro	VRT-125853 (32 μg/ml)	Novo (0.5 μg/ml)
S. aureus ATCC 29213 GyrB	WT	No plasmid	2	0.06	0.13	0.25
	WT	WT	2	0.06	0.13	0.25
	T173N	No plasmid	8	0.25	0.25	0.25
	WT	T173N	8	0.25	0.13	0.25
	T173I	No plasmid	8	0.25	2	0.25
	WT	T173I	8	0.13	0.5	0.25
	R144I	No plasmid	2	0.06	8	0.25
	WT	R144I	2	0.06	8	0.25
H. influenzae ATCC 51907 GyrB	WT						0	0
	T169I						71	ND
	R140C						ND	141
	R140H						ND	53
	R140L						ND	245

^aAbbreviations: WT, wild type; Novo, novobiocin; Cipro, ciprofloxacin; ND, not determined.