TABLE 1.
EC50 values for DFMO, MDL 73811, and n-butylaminea
| Strain | Mean EC50 (mM) ± SD
|
||
|---|---|---|---|
| DFMO | MDL73811 | n-Butylamine | |
| Wild type | 0.04 ± 0.02 | 0.04 ± 0.02 | 0.4 ± 0.3 |
| DI700[pODC] | >1 | 2.7 ± 0.5 | 2.1 ± 0.4 |
| DI700[pSPDSYN] | 0.04 ± 0.01 | 0.2 ± 0.1 | 3.3 ± 0.8 |
| DI700[pADOMETDC] | 0.03 ± 0.02 | >10 | 0.2 ± 0.07 |
| Wild type + putrescine | >1 | 0.8 ± 0.2 | 2.0 ± 0.4 |
| Wild type + spermidine | 0.13 ± 0.08 | >10 | >10 |
a
The EC50 values for wild-type parasites, wild-type parasites grown in the presence of 100 μM putrescine or 100 μM spermidine, and ADOMETDC-, ODC-, or SPDSYN-overproducing parasites were determined. Each value is the mean and standard deviation of three independent experiments.