FIG. 3.
Time courses of resistance mutation selection for VRX-480773 and efavirenz. Wild-type NL4.3 was used to infect SupT1 cells (2 × 106 cells in 1 ml of RPMI 1640 medium containing 10% fetal bovine serum) in the initial presence of 1 nM VRX-480773 or 2 nM efavirenz. Subsequently, every 3 to 4 days, 100 μl of infected culture was transferred into 900 μl of medium containing fresh drug and 9 × 105 SupT1 cells. Virus replication was monitored microscopically by observing the formation of syncytia. At each virus breakthrough (massive syncytium formation), the concentration of the inhibitor was doubled.